Equine sperm concentration for artificial insemination
Looking to breed with chilled semen? There are many benefits but unfortunately, not all stallions produce sperm that survive the cooling process. Here, the American Association of Equine Practitioners outlines the process of breeding horses with cooled semen. Stallions should be tested before the breeding season to determine if sperm motility will be acceptable when mares are bred with cooled transported semen. Management of the mare should be precise and intense in order to reduce expenses and maximize fertility associated with breeding with cooled, transported semen.
Quality and fertility of cooled-shipped stallion semen at the time of insemination
The target of semen evaluation is to clarify if quantitative and qualitative spermatological parameters are in compliance with the minimum requirements fixed for biological composition of stallion semen. Minimum standards are empirically determined averages with consideration of the standard deviation of spermatological parameters in a fertile stallion population. To define standards, variation, and minimal criteria of stallion semen, Klug 11 and Parlevliet et al. Available at www. The predictive value of semen analysis in the evaluation of stallion fertility has been reported by numerous investigators.
In the beginning, there was tremendous variability in the fertility of semen shipped throughout the country and subsequent pregnancy rates. Fortunately, many advances have been made in the technology of shipped semen as well as improved training of personnel responsible for the collection, processing and shipment of cooled stallion semen. Still, the basics for success have not changed. Pregnancy rates of mares bred with cooled shipped semen are dependent on three variables:. First, the experience of the veterinarian who not only coordinates the timing of insemination with ovulation, but also evaluates and processes the stallion semen for shipment, is paramount to success.
One of the challenges faced by equine breeders is ensuring delivery of good quality semen doses for artificial insemination when the mare is due to ovulate. Single Layer Centrifugation SLC has been shown to select morphologically normal spermatozoa with intact chromatin and good progressive motility from the rest of the ejaculate, and to prolong the life of these selected spermatozoa in vitro. The objective of the present study was a proof of concept, to determine whether fertilizing ability was retained in SLC-selected spermatozoa during prolonged storage. However, there can be problems with delivery of cooled semen on the day when it is required, particularly over the weekends and during public holidays.